Exercise protocols and analytical procedures for assessing the heart rate deflection point in athletes: a systematic review
DOI:
https://doi.org/10.24310/riccafd.14.1.2025.21060Keywords:
exercise physiology, anaerobic threshold , sportsAbstract
Introduction: This systematic review evaluates the exercise protocols used to determine the heart rate deflection point (HRDP) in athletes, emphasizing its relevance as a non-invasive biomarker of the anaerobic threshold. Methods: A systematic search was conducted in EBSCOhost, PubMed, and Scopus from 1982 to May 2024 using terms such as "heart rate deflection point" and "anaerobic threshold." Original studies including athletes of any age, sex, and competitive level were included, while low-quality studies and duplicate data were excluded. Extracted data included participant characteristics, exercise protocols (e.g., ramp tests, Conconi protocols), and HRDP assessment methods (visual or computational). Results: A total of 31 studies involving 3,522 athletes (91.8% men) were included. The results showed significant variability in HRDP identification depending on the protocol used. Ramp tests and Conconi protocols (original and modified) achieved identification rates above 90% in specific populations, whereas field-based methods demonstrated lower sensitivity (<50%). Reproducibility and validity of HRDP measurements also varied according to protocol design, participant characteristics, and analytical techniques. Conclusions: While HRDP is a promising tool for estimating the anaerobic threshold, its practical application is limited by methodological inconsistencies. Future research is needed to establish standardized and reproducible protocols
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Petek, Bradley J et al. “Cardiopulmonary Exercise Testing in Athletes: Expect the Unexpected.” Current treatment options in cardiovascular medicine vol. 23,7 (2021): 49. doi:10.1007/s11936-021-00928-z.
Chávez-Guevara, Isaac A et al. “Difficult but not impossible: paving the way to standardization in the assessment of exercise physiology thresholds.” The Journal of physiology vol. 602,19 (2024): 5107-5108. doi:10.1113/JP287380.
Poole, David C et al. “The anaerobic threshold: 50+ years of controversy.” The Journal of physiology vol. 599,3 (2021): 737-767. doi:10.1113/JP279963
Faude, Oliver et al. “Lactate threshold concepts: how valid are they?.” Sports medicine (Auckland, N.Z.) vol. 39,6 (2009): 469-90. doi:10.2165/00007256-200939060-00003.
MacIntosh, Brian R et al. “What Is Moderate to Vigorous Exercise Intensity?.” Frontiers in physiology vol. 12 682233. 22 Sep. 2021, doi:10.3389/fphys.2021.682233.
Binder, Ronald K et al. “Methodological approach to the first and second lactate threshold in incremental cardiopulmonary exercise testing.” European journal of cardiovascular prevention and rehabilitation: official journal of the European Society of Cardiology, Working Groups on Epidemiology & Prevention and Cardiac Rehabilitation and Exercise Physiology vol. 15,6 (2008): 726-34. doi:10.1097/HJR.0b013e328304fed4.
Vucetić V, Sentija D, Sporis G, Trajković N, Milanović Z. Comparison of ventilation threshold and heart rate deflection point in fast and standard treadmill test protocols. Acta Clin Croat. 2014;53(2):190–203.
Yuen WK, Schreiner SR, Hoover DL, Loudon JK, Billinger SA. Does the Friel Anaerobic Threshold Test Accurately Detect Heart Rate Deflection in Trained Cyclists. International journal of exercise science. 2011;4(3):164–75.
Bodner ME, Rhodes EC. A review of the concept of the heart rate deflection point. Sports Med [Internet]. 2000;30(1):31–46. Disponible en: http://dx.doi.org/10.2165/00007256-200030010-00004.
Conconi F, Ferrari M, Ziglio PG, Droghetti P, Codeca L. Determination of the anaerobic threshold by a noninvasive field test in runners. J Appl Physiol [Internet]. 1982;52(4):869–73. Disponible en: http://dx.doi.org/10.1152/jappl.1982.52.4.869.
Conconi F, Grazzi G, Casoni I, Guglielmini C, Borsetto C, Ballarin E, et al. The Conconi test: methodology after 12 years of application. Int J Sports Med [Internet]. 1996;17(7):509–19. Disponible en: http://dx.doi.org/10.1055/s-2007-972887.
Jones AM, Doust JH. Lack of reliability in Conconi’s heart rate deflection point. Int J Sports Med [Internet]. 1995;16(8):541–4. Disponible en: http://dx.doi.org/10.1055/s-2007-973051.
Birnbaumer P, Dostal T, Cipryan L, Hofmann P. Pattern of the heart rate performance curve in maximal graded treadmill running from 1100 healthy 18-65 Years old men and women: the 4HAIE study. Front Physiol. 2023;14:1178913. Published 2023 May 30. doi:10.3389/fphys.2023.1178913.
Buchheit M, Solano R, Millet GP. Heart-rate deflection point and the second heart-rate variability threshold during running exercise in trained boys. Pediatr Exerc Sci [Internet]. 2007;19(2):192–204. Disponible en: http://dx.doi.org/10.1123/pes.19.2.192.
Guimarães MP, Campos YAC, Souza HLR, Da Silva SF. Is There a Concordance between the Lactate Threshold and the Heart Rate Deflection Point during a Progressive Field Test in Moderately Trained Distance Runners? JEPonline 2017;20(5): 60-68.
Page, Matthew J et al. “The PRISMA 2020 statement: an updated guideline for reporting systematic reviews.” BMJ (Clinical research ed.) vol. 372 n71. 29 Mar. 2021, doi:10.1136/bmj.n71.
Cohen, C., & Muehl, G. (1977). Human circadian rhythms in resting and exercise pulse rates.. Ergonomics, 20 5, 475-9. https://doi.org/10.1080/00140137708931656.
Kara M, Gökbel H, Bedìz C, Ergene N, Uçok K, Uysal H. Determination of the heart rate deflection point by the Dmax method. J Sports Med Phys Fitness. 1996;36(1):31–4.
Bourgois J, Coorevits P, Danneels L, Witvrouw E, Cambier D, Vrijens J. Validity of the heart rate deflection point as a predictor of lactate threshold concepts during cycling. J Strength Cond Res [Internet]. 2004;18(3):498–503. Disponible en: http://dx.doi.org/10.1519/00124278-200408000-00018.
Doston CO, Humphrey JH. Exercise physiology: current selected research external validity of the conconi’s heart rate anaerobic threshold as compared to the lactate threshold. 1988;3.
Petit MA, Nelson CM, Rhodes EC. Comparison of a mathematical model to predict 10-km performance from the Conconi test and ventilatory threshold measurements. Can J Appl Physiol [Internet]. 1997;22(6):562–72. Disponible en: http://dx.doi.org/10.1139/h97-036.
Bourgois J, Vrijens J. The Conconi test: a controversial concept for the determination of the anaerobic threshold in young rowers. Int J Sports Med [Internet]. 1998;19(8):553–9. Disponible en: http://dx.doi.org/10.1055/s-2007-971959.
Schmid A, Huonker M, Aramendi JF, Klüppel E, Barturen JM, Grathwohl D, et al. Heart rate deflection compared to 4 mmol x l(-1) lactate threshold during incremental exercise and to lactate during steady-state exercise on an arm-cranking ergometer in paraplegic athletes. European Journal of Applied Physiology and Occupational Physiology. 1998;78(2):177–82.
Lucía A, Carvajal A, Boraita A, Serratosa L, Hoyos J, Chicharro JL. Heart dimensions may influence the occurrence of the heart rate deflection point in highly trained cyclists. Br J Sports Med [Internet]. 1999;33(6):387–92. Disponible en: http://dx.doi.org/10.1136/bjsm.33.6.387.
Vachon JA, Bassett DR Jr, Clarke S. Validity of the heart rate deflection point as a predictor of lactate threshold during running. J Appl Physiol [Internet]. 1999;87(1):452–9. Disponible en: http://dx.doi.org/10.1152/jappl.1999.87.1.452.
Bodner ME, Rhodes EC, Martin AD, Coutts KD. The relationship of the heart rate deflection point to the ventilatory threshold in trained cyclists. J Strength Cond Res [Internet]. 2002;16(4):573–80. Disponible en: http://dx.doi.org/10.1519/00124278-200211000-00014.
Carey, Daniel G et al. “Intra- and inter-observer reliability in selection of the heart rate deflection point during incremental exercise: comparison to a computer-generated deflection point.” Journal of sports science & medicine vol. 1,4 115-21. 1 Dec. 2002.
Lucía A, Hoyos J, Santalla A, Pérez M, Carvajal A, Chicharro JL. Lactic acidosis, potassium, and the heart rate deflection point in professional road cyclists. Br J Sports Med [Internet]. 2002;36 (2):113–7. Disponible en: http://dx.doi.org/10.1136/bjsm.36.2.113.
Laursen PB, Knez WL, Shing CM, Langill RH, Rhodes EC, Jenkins DG. Relationship between laboratory-measured variables and heart rate during an ultra-endurance triathlon. J Sports Sci [Internet]. 2005;23(10):1111–20. Disponible en: http://dx.doi.org/10.1080/02640410400023209.
Hofmann P, Pokan R, von Duvillard SP, Seibert FJ, Zweiker R, Schmid P. Heart rate performance curve during incremental cycle ergometer exercise in healthy young male subjects. Med Sci Sports Exerc [Internet]. 1997;29(6):762–8. Disponible en: http://dx.doi.org/10.1097/00005768-199706000-00005
Sentija D, Vucetic V, Markovic G. Validity of the modified Conconi running test. Int J Sports Med [Internet]. 2007;28(12):1006–11. Disponible en: http://dx.doi.org/10.1055/s-2007-965071.
Grazzi G, Mazzoni G, Casoni I, Uliari S, Collini G, Heide L van der, et al. Identification of a JOURNAL/jscr/04.03/00124278-200807000-00013/OV0312_4/v/2021-02-09T093601Z/r/image-png o2 deflection point coinciding with the heart rate deflection point and ventilatory threshold in cycling. J Strength Cond Res [Internet]. 2008;22(4):1116–23. Disponible en: http://dx.doi.org/10.1519/jsc.0b013e318173936c.
Fabre N, Passelergue P, Bouvard M, Perrey S. Comparison of heart rate deflection and ventilatory threshold during a field cross-country roller-skiing test. J Strength Cond Res [Internet]. 2008;22(6):1977–84. Disponible en: http://dx.doi.org/10.1519/JSC.0b013e3181874ae9.
Cabo JV, Martinez-Camblor P, Del Valle M. Validity of the modified conconi test for determining ventilatory threshold during on-water rowing. J Sports Sci Med. 2011;10(4):616-623. Published 2011 Dec 1.
Marques-Neto SR, Maior AS, Maranhão Neto GA, Santos EL. Analysis of heart rate deflection points to predict the anaerobic threshold by a computerized method. J Strength Cond Res [Internet]. 2012;26(7):1967–74. Disponible en: http://dx.doi.org/10.1519/JSC.0b013e318234eb5d.
Vitor B, Nascimento R, Osiecki PE, Redkva EB, Gomes FM, Salgueirosa LB, et al. Identification and Concordance of the Heart Rate Deflection Point and Heart Rate Variability Threshold Determined by Different Parameters in the 20 m Multistage Fitness Test. Official Research Journal of the American Society of Exercise Physiologists. 2012.
Baiget E, Fernández-Fernández J, Iglesias X, Rodríguez FA. Heart rate deflection point relates to second ventilatory threshold in a tennis test. J Strength Cond Res [Internet]. 2015;29(3):765–71. Disponible en: http://dx.doi.org/10.1519/JSC.0000000000000664.
Silva D, Peserico CS, Machado FA. Relationship between heart rate deflection point determined by Dmax method and 10-km running performance in endurance recreationally- trained female runners. The Journal of Sports Medicine and Physical Fitness. 2015.
Kjertakov M, Dalip M, Hristovski R, Epstein Y. Prediction of lactate threshold using the modified Conconi test in distance runners. Physiol Int [Internet]. 2016;103(2):262–70. Disponible en: http://dx.doi.org/10.1556/036.103.2016.2.12
Costa PL, Conde JHS, Silva JKF, Favaro, SVB, De Oliveira FR, Osiecki R. Relationship between Heart Rate Deflection Point and Ventilatory Threshold in Elite Mountain Runners. JEPonline 2017;20(4):184-191.
Etxegarai U, Insunza A, Larruskain J, Santos-Concejero J, Gil SM, Portillo E, et al. Prediction of performance by heart rate-derived parameters in recreational runners. J Sports Sci [Internet]. 2018;36(18):2129–37. Disponible en: http://dx.doi.org/10.1080/02640414.2018.1442185.
Hnizdil J, Skopek M, Nosek M, Louka O, Musalek M, Heller J. The Conconi Test – Searching for the Deflection Point. Physical Activity Review [Internet]. 2019;7:160–7. Available from: http://dx.doi.org/10.16926/par.2019.07.19.
De Lima P. Relationship between Heart Rate Deflection Point and Ventilatory Threshold in Elite Mountain Runners. Journal of Exercise Physiology. 2017.
Lepretre PM, Foster C, Koralsztein JP, Billat VL. Heart rate deflection point as a strategy to defend stroke volume during incremental exercise. J Appl Physiol (1985). 2005;98(5):1660-1665. doi:10.1152/japplphysiol.00837.2004.
Beck KC, Randolph LN, Bailey KR, Wood CM, Snyder EM, Johnson BD. Relationship between cardiac output and oxygen consumption during upright cycle exercise in healthy humans. J Appl Physiol (1985). 2006;101(5):1474-1480. doi:10.1152/japplphysiol.00224.2006.
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